Purification of Islets of Langerhans from Porcine Pancreas
نویسندگان
چکیده
منابع مشابه
Preparation of islets of Langerhans from the hamster pancreas.
We describe a method for the preparation of viable islets from the pancreas of normal 8-week-old female Syrian golden hamster, based on the injection of the pancreatic duct with collagenase and on mechanical dissociation which liberates islets that maintain their normal morphological appearance and physiologic function. In a series of 11 animals, we examined the dose response of intraductal col...
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Co-lipase has been purified from porcine pancreas by a method involving tile following steps: acid extraction of pancreas homogenate, heat treatment, (NH4)2SO 4 fractionation, t reatment with ethanol, chromatography on SP-Sephadex, chromatography on DEAE-cellulose and filtration through Sephadex G-75. The method can be applied on a relatively large preparative scale. Starting from 5oo g of panc...
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آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
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BACKGROUND Ischemia/reperfusion (I/R) injury plays a pivotal role in the development of graft pancreatitis, with ischemia time representing one of its crucial factors. However, it is unclear, whether exocrine and endocrine tissue experience similar inflammatory responses during pancreas transplantation (PTx). This study evaluated inflammatory susceptibilities of islets of Langerhans (ILH) and e...
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ژورنال
عنوان ژورنال: The Scientific World JOURNAL
سال: 2002
ISSN: 1537-744X
DOI: 10.1100/tsw.2002.847